中文版 | English
CSpace
A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle
Yang, Yun1; Wang, Hao1; He, Jia1; Shi, Wenchao1; Jiang, Zhanmei1; Gao, Lina1; Jiang, Yan1; Ni, Rui1; Yang, Qifen1; Luo, Lingfei1,2
2021-06-22
摘要A progenitor cell could generate a certain type or multiple types of descendant cells during embryonic development. To make all the descendant cell types and developmental trajectories of every single progenitor cell clear remains an ultimate goal in developmental biology. Characterizations of descendant cells produced by each uncommitted progenitor for a full germ layer represent a big step toward the goal. Here, we focus on early foregut endoderm, which generates foregut digestive organs, including the pancreas, liver, foregut, and ductal system, through distinct lineages. Using unbiased single-cell labeling techniques, we label every individual zebrafish foregut endodermal progenitor cell out of 216 cells to visibly trace the distribution and number of their descendant cells. Hence, single-cell-resolution fate and proliferation maps of early foregut endoderm are established, in which progenitor regions of each foregut digestive organ are precisely demarcated. The maps indicate that the pancreatic endocrine progenitors are featured by a cell cycle state with a long G1 phase. Manipulating durations of the G1 phase modulates pancreatic progenitor populations. This study illustrates foregut endodermal progenitor cell fate at single-cell resolution, precisely demarcates different progenitor populations, and sheds light on mechanistic insights into pancreatic fate determination.
关键词single-cell labeling and tracing endoderm fate map pancreatic fate cell cycle
DOI10.1073/pnas.2025793118|1of9
发表期刊PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN0027-8424
卷号118期号:25页码:9
通讯作者Luo, Lingfei(lluo@swu.edu.cn)
收录类别SCI
WOS记录号WOS:000675808200014
语种英语