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Enzyme inspired polymer functionalized with an artificial catalytic triad
Bhaskaran, Ayana1; Aitken, Heather M.1; Xiao, Zeyun2,3; Blyth, Mitchell1; Nothling, Mitchell D.2; Kamdar, Shashank2; O'Mara, Megan L.1; Connal, Luke A.1
2021-05-26
摘要At the heart of an enzyme's structure is the active catalytic site, which, together with the surrounding amino-acid residues, tunes the substrate properties in the electrostatic microenvironment. Taking inspiration from nature's catalytic triad, consisting of a hydroxyl group of a serine residue, an imidazole group of a histidine residue, and a carboxyl group of an aspartic acid, a polymer scaffolded enzyme mimic is incorporated with artificial catalytic triad units. It is demonstrated that controlling the local environment of the artificial triad affects catalytic activity. Compared to a water soluble artificial catalytic triad, enhanced esterolytic activity of p-nitrophenyl benzoate was observed for the polymeric catalyst possibly due to the hydrophobic microenvironment formed by the tertiary structure of the polymer backbone. The different environments were supported by molecular dynamics simulations. It is further demonstrated that the cross-linked catalytic triad functional polystyrene could be used as a reusable solid support catalyst for esterolysis.
关键词Enzyme mimic Bioinspired Catalyst
DOI10.1016/j.polymer.2021.123735
发表期刊POLYMER
ISSN0032-3861
卷号225页码:5
通讯作者Connal, Luke A.(Luke.connal@anu.edu.au)
收录类别SCI
WOS记录号WOS:000651460700007
语种英语