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Terahertz, infrared and Raman absorption spectra of tyrosine enantiomers and racemic compound
Hu, Meidie1,2; Tang, Mingjie2,3,4; Wang, Huabin2,3,4; Zhang, Mingkun2,3,4; Zhu, Shiping1; Yang, Zhongbo2,3,4; Zhou, Shengling1; Zhang, Hua2,3,4; Hu, Jiao2; Guo, Yuansen2
2021-06-05
摘要The application of terahertz (THz)-based techniques in biomolecule study is very promising but still in its infancy. In the present work, we employed THz time-domain spectroscopy (THz-TDS) and THz time-domain attenuated total reflection (THz-TD-ATR) spectroscopy to investigate the properties of tyrosine (Tyr) enantiomers (L-and D-Tyr) and racemate (DL-Tyr) in solid state and aqueous solutions, respec-tively. THz absorption spectra of solid L-and D-Tyr show similar absorption spectra with peaks at 0.95, 1.92, 2.06 and 2.60 THz, which are obviously different from the spectrum of DL-Tyr with peaks at 1.5, 2.15 and 2.40 THz. In contrast, although THz absorption spectra of L-Tyr solution and D-Tyr solution are similar and different from the spectrum of DL-Tyr solution, both of them have no observable peaks. Interestingly, it was found that the solution containing equal amounts of L-and D-Tyr has a similar spec-trum as that of DL-Tyr solution, as far as the mass concentrations of the two types of solutions are kept the same. On other hand, solid L-, D-and DL-Tyr were also investigated with infrared spectroscopy and Raman spectroscopy, respectively. The results show that the spectra of L-and D-Tyr can be regarded the same but they are slightly different from the spectrum of DL-Tyr. With the aid of principal component analysis (PCA), the difference between L-/D-Tyr and DL-Tyr can be confirmed without any ambiguity. Overall, this work systematically interrogated and evaluated the performance of THz-based techniques in the detection of the chirality of tyrosine. (c) 2021 Elsevier B.V. All rights reserved.
关键词Tyrosine Terahertz time-domain spectroscopy Attenuated total reflection Chirality Infrared spectroscopy Raman spectroscopy
DOI10.1016/j.saa.2021.119611
发表期刊SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
ISSN1386-1425
卷号254页码:8
通讯作者Wang, Huabin(wanghuabin@cigit.ac.cn) ; Zhu, Shiping(zspswu@126.com)
收录类别SCI
WOS记录号WOS:000639679500001
语种英语