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Recognition of Bimolecular Logic Operation Pattern Based on a Solid-State Nanopore
Yan, Han1; Zhang, Zhen1; Weng, Ting2; Zhu, Libo1; Zhang, Pang2; Wang, Deqiang2; Liu, Quanjun1
2021
摘要Nanopores have a unique advantage for detecting biomolecules in a label-free fashion, such as DNA that can be synthesized into specific structures to perform computations. This method has been considered for the detection of diseased molecules. Here, we propose a novel marker molecule detection method based on DNA logic gate by deciphering a variable DNA tetrahedron structure using a nanopore. We designed two types of probes containing a tetrahedron and a single-strand DNA tail which paired with different parts of the target molecule. In the presence of the target, the two probes formed a double tetrahedron structure. As translocation of the single and the double tetrahedron structures under bias voltage produced different blockage signals, the events could be assigned into four different operations, i.e., (0, 0), (0, 1), (1, 0), (1, 1), according to the predefined structure by logic gate. The pattern signal produced by the AND operation is obviously different from the signal of the other three operations. This pattern recognition method has been differentiated from simple detection methods based on DNA self-assembly and nanopore technologies.
关键词nanopore DNA logic gate DNA tetrahedron probe AND operation
DOI10.3390/s21010033
发表期刊SENSORS
卷号21期号:1页码:11
通讯作者Liu, Quanjun(lqj@seu.edu.cn)
收录类别SCI
WOS记录号WOS:000606130500001
语种英语