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Potassium regulates the growth and toxin biosynthesis of Microcystis aeruginosa
He, Yixin1; Ma, Jianrong2; Joseph, Vanderwall3; Wei, Yanyan4; Liu, Mengzi1; Zhang, Zhaoxue1; Li, Guo1; He, Qiang1; Li, Hong1
2020-12-01
摘要Potassium (K+) is the most abundant cation in phytoplankton cells, but its impact on Microcystis aeruginosa (M. aeruginosa) has not been fully documented. This study presents evidence of how K+ availability affects the growth, oxidative stress and microcystin (MC) production of M. aeruginosa. The iTRAQbased proteomic analysis revealed that during K+ deficiency, serious oxidative damage occurred and the photosynthesis-associated and ABC transporter-related proteins in M. aeruginosa were substantially downregulated. In the absence of K+, a 69.26% reduction in cell density was shown, and both the photosynthesis and iron uptake were depressed, which triggered a declined production of ATP and expression of MC synthetases genes (mcyA, B and D), and MC exporters (mcyH). Through the impairment of both the MC biosynthesis and MC transportation out of cells, K+ depletion caused an 85.89% reduction of extracellular MC content at the end of the study. However, with increasing in the available K+ concentrations, photosynthesis efficiency, the expression of ABC-transporter proteins, and the transcription of mcy genes displayed slight differences compared with those in the control group. This work represents evidence that K+ availability can regulate the physiological metabolic activity of M. aeruginosa and K+ deficiency leads to depressed growth and MC production in M. aeruginosa. (C) 2020 Elsevier Ltd. All rights reserved.
关键词Potassium Microcystis iTRAQ Proteomic Microcystin
DOI10.1016/j.envpol.2020.115576
发表期刊ENVIRONMENTAL POLLUTION
ISSN0269-7491
卷号267页码:11
通讯作者Li, Hong(hongli@cqu.edu.cn)
收录类别SCI
WOS记录号WOS:000593967000017
语种英语