KMS Chongqing Institute of Green and Intelligent Technology, CAS
| Near-Field Nanoscopic Terahertz Imaging of Single Proteins | |
Yang, Zhongbo1,2,3 ; Tang, Dongyun1,2,3; Hu, Jiao1,2,3; Tang, Mingjie1,2,3; Zhang, Mingkun1,2,3; Cui, Hong-Liang1,2; Wang, Lihua4; Chang, Chao5; Fan, Chunhai6,7; Li, Jiang4
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| 2020-12-11 | |
| 摘要 | Terahertz (THz) biological imaging has attracted intense attention due to its capability of acquiring physicochemical information in a label-free, noninvasive, and nonionizing manner. However, extending THz imaging to the single-molecule level remains a challenge, partly due to the weak THz reflectivity of biomolecules with low dielectric constants. Here, the development of graphene-mediated THz scattering-type scanning near-field optical microscope for direct imaging of single proteins is reported. Importantly, it is found that a graphene substrate with high THz reflectivity and atomic flatness can provide high THz contrast against the protein molecules. In addition, a platinum probe with an optimized shaft length is found enabling the enhancement of the amplitude of the scattered THz near-field signals. By coupling these effects, the topographical and THz scattering images of individual immunoglobulin G (IgG) and ferritin molecules with the size of a few nanometers are obtained, simultaneously. The demonstrated strategy thus opens new routes to imaging single biomolecules with THz. |
| 关键词 | graphene HOPG near‐ field microscope single biomolecules terahertz |
| DOI | 10.1002/smll.202005814 |
| 发表期刊 | SMALL
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| ISSN | 1613-6810 |
| 页码 | 6 |
| 通讯作者 | Li, Jiang(lijiang@zjlab.org.cn) ; Wang, Huabin(wanghuabin@cigit.ac.cn) |
| 收录类别 | SCI |
| WOS记录号 | WOS:000597868700001 |
| 语种 | 英语 |
