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Effects of Exogenous Organic Acids on Cd Tolerance Mechanism of Salix variegata Franch. Under Cd Stress
Zhang, Songlin1,2; Chen, Hongchun1,3; He, Danni1; He, Xinrui1; Yan, Ya3; Wu, Kejun1; Wei, Hong1
2020-10-23
摘要Chelate induction of organic acids has been recognized to enhance metal uptake and translocation in plants, but the underlying mechanism remains unclear. In this study, seedlings of Salix variegata were hydroponically exposed to the combinations of Cd (0 and 50 mu M) and three exogenous organic acids (100 mu M of citric, tartaric, or malic acid). Plant biomass, antioxidant enzymes, non-protein thiol compounds (NPT) content, and the expression of candidate genes associated with Cd accumulation and tolerance were determined. Results showed that Cd significantly inhibited plant biomass but stimulated the activity of antioxidant enzymes in the roots and leaves, while the lipid peroxidation increased as well. Respective addition of three organic acids greatly enhanced plant resistance to oxidative stress and reduced the lipid peroxidation induced by Cd, with the effect of malic acid showing greatest. The addition of organic acids also significantly increased the content of glutathione in the root, further improving the antioxidant capacity and potential of phytochelatin biosynthesis. Moreover, Cd induced the expression level of candidate genes in roots of S. variegata. The addition of three organic acids not only promoted the expression of candidate genes but also drastically increased Cd accumulation in S. variegata. In summary, application of citric, tartaric, or malic acid alleviated Cd-imposed toxicity through the boost of enzymatic and non-enzymatic antioxidants and candidate gene expression, while their effects on Cd tolerance and accumulation of S. variegata differed.
关键词cadmium Salix variegata organic acid antioxidant enzymatic system non-protein sulfhydryl compounds gene expression
DOI10.3389/fpls.2020.594352
发表期刊FRONTIERS IN PLANT SCIENCE
ISSN1664-462X
卷号11页码:13
通讯作者Wei, Hong(weihong@swu.edu.cn)
收录类别SCI
WOS记录号WOS:000587015100001
语种英语