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	Detection of single-base mutation of DNA oligonucleotides with different lengths by terahertz attenuated total reflection microfluidic cell
	Tang, Mingjie1,2; Zhang, Mingkun1; Xia, Liangping1,4; Yang, Zhongbo1; Yan, Shihan1; Wang, Huabin1; Wei, Dongshan1,3; Du, Chunlei1; Cui, Hong-Liang1
	2020-09-01
摘要	Many human genetic diseases are caused by single-base mutation in the gene sequence. Since DNA molecules with single-base mutation are extremely difficult to differentiate, existing detection methods are invariably complex and time-consuming. We propose a new label-free and fast terahertz (THz) spectroscopic technique based on a home-made terahertz attenuated total reflection (ATR) microfluidic cell and a terahertz time-domain spectroscopy (THz-TDS) system to detect single-base-mutated DNA molecules. The detected object DNA molecules are normal hemoglobin gene, sickle cell anemia gene (15 nt), JAK2 gene wild type and JAK2 V617F gene mutation (39 nt) from sickle cell anemia and thrombocytopenia, respectively. Results show that the oligonucleotide fragments with single-base mutation can be identified by THz spectroscopy combined with the ATR microfluidic cell, and the recognition effect of short oligonucleotide fragments with single-base mutation is better than that of long oligonucleotide fragments. The terahertz biosensor is shown to have high sensitivity and can be used to detect DNA molecules directly in the solution environment. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
DOI	10.1364/BOE.400487
发表期刊	BIOMEDICAL OPTICS EXPRESS
ISSN	2156-7085
卷号	11期号:9页码:5362-5372
通讯作者	Wei, Dongshan(dswei@dgut.edu.cn)
收录类别	SCI
WOS记录号	WOS:000577455500036
语种	英语