KMS Chongqing Institute of Green and Intelligent Technology, CAS
| Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum | |
Su, HaiFeng1,2 ; Chen, Hua4; Lin, JiaFu3
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| 2020-04-16 | |
| 摘要 | Non-natural 2-methyl-1-butanol (2 MB) has been biosynthesized through the modification of metabolic pathways using Corynebacterium crenatum, a non-model host. However, its production capacity is not effectively improved. In this study, the fermentation process was strengthened through factor combination design (FCD) for enhancing the production of 2 MB. Our results showed that the highest production of 2 MB, 3-methyl-1-butanol (3 MB), ethanol, and total solvent was 4.87 +/- 0.39 g/L, 3.57 +/- 0.21 g/L, 5.74 +/- 0.43 g/L, and 14.18 g/L, respectively, under the optimal fermentation conditions. The optimal fermentation conditions were determined through the FCD to be as follows: pH of 6.5, IPTG concentration of 1.2 mM, fermentation temperature of 32 degrees C, and fermentation time of 96 h. This study provides a significant guidance for the optimal control technology of the genetically engineered C. crenatum, and also a useful reference for the industrial production of 2 MB via the microbial fermentation approach. |
| DOI | 10.1007/s00284-020-01961-0 |
| 发表期刊 | CURRENT MICROBIOLOGY
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| ISSN | 0343-8651 |
| 页码 | 8 |
| 通讯作者 | Lin, JiaFu(linjiafu@cdu.edu.cn) |
| 收录类别 | SCI |
| WOS记录号 | WOS:000533859200004 |
| 语种 | 英语 |
