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Single cell imaging with near-field terahertz scanning microscopy
Li, Zaoxia1,2,3; Yan, Shihan1,2; Zang, Ziyi1,2,3; Geng, Guoshuai1,2,3; Yang, Zhongbo1,2; Li, Jiang4,5; Wang, Lihua4,5; Yao, Chunyan6; Cui, Hong-Liang1,2,3; Chang, Chao7,8
2020-04-01
摘要Objectives Terahertz (THz)-based imaging techniques hold great potential for biological and biomedical applications, which nevertheless are hampered by the low spatial resolution of conventional THz imaging systems. In this work, we report a high-performance photoconductive antenna microprobe-based near-field THz time-domain spectroscopy scanning microscope. Materials and methods A single watermelon pulp cell was prepared on a clean quartz slide and covered by a thin polyethylene film. The high performance near-field THz microscope was developed based on a coherent THz time-domain spectroscopy system coupled with a photoconductive antenna microprobe. The sample was imaged in transmission mode. Results We demonstrate the direct imaging of the morphology of single watermelon pulp cells in the natural dehydration process with our near-field THz microscope. Conclusions Given the label-free and non-destructive nature of THz detection techniques, our near-field microscopy-based single-cell imaging approach sheds new light on studying biological samples with THz.
关键词dehydration near-field imaging photoconductive antenna microprobe scanning microscope single cell terahertz
DOI10.1111/cpr.12788
发表期刊CELL PROLIFERATION
ISSN0960-7722
卷号53期号:4页码:7
通讯作者Yao, Chunyan(yaochunyan@tmmu.edu.cn) ; Wang, Huabin(wanghuabin@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:000528669500004
语种英语