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A self-assembled nanocompartment in anammox bacteria for resisting intracelluar hydroxylamine stress
Xing, Chong-Yang1,2; Fan, Yu-Chen3; Chen, Xuan3; Guo, Jin-Song3; Shen, Yu4; Yan, Peng3; Fang, Fang3; Chen, You-Peng1,2,3
2020-05-15
摘要Anammox bacteria play an important role in the global nitrogen cycle, but research on anammoxosome structure is still in its initial stages. In particular, the anammox bacteria genome contains nanocompartments gene loci. However, the function and structure of the nanocompartments in anammox bacteria is poorly understood. We apply genetic engineering to demonstrate the self-assembled nanocompartments of anammox bacteria. The encapsulin shell protein (cEnc) and cargo protein hydroxylamine oxidoreductase (HAO) can self-assemble to form regular nanocompartments (about 128 nm in diameter) in vitro. Cell growth curve tests show that nanocompartments help model bacteria resist hydroxylamine (NH2OH) stress. Batch test results and transcriptional data show that cEnc and HAO are highly expressed in response to the negative effects of NH2OH on anammox efficiency, predicting a potential role of nanocompartments in helping anammox bacteria resist NH2OH stress. These findings improve our understanding of the mechanisms by which anammox bacteria respond to harmful environmental metabolites. (C) 2020 Elsevier B.V. All rights reserved.
关键词Anammox Nanocompartment Hydroxylamine Hydroxylamine oxidoreductase Encapsulin shell protein
DOI10.1016/j.scitotenv.2020.137030
发表期刊SCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
卷号717页码:8
通讯作者Chen, You-Peng(ypchen@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:000519994800013
语种英语