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Probing the changes of carotenoids in Microcystis flosaquae under environmental perturbations by two-dimensional Raman correlation spectroscopy
He, Shixuan1,2; Xie, Wanyi1; Fang, Shaoxi1; Zhang, Ping3; Li, Zhe1; Zhou, Daming1; Zhang, Yongna1; Zhang, Zhiyou2; Guo, Jinsong3; Du, Jinglei2
2019-11-12
摘要Raman mapping spectra of Microcystis flosaquae (M. flosaquae) subjected to environmental perturbations were used to study the correlations among the characteristics of M. flosaquae by two-dimensional (2-D) Raman correlation spectroscopy. Overlapping Raman characteristics of the carotenoids in M. flosaquae and their differences in algal growths were shown in the 2-D synchronous and asynchronous correlation spectra. The asynchronous correlation Raman spectra demonstrated the accumulation of carotenoids in M. flosaquae under normal culture condition and environmental perturbations. High light flux obviously led to the accumulation of beta-carotene in M. flosaquae in comparison with normal culture condition. The results showed that 2-D Raman correlation spectroscopy can be used to analyze the correlation of complex changes of the Raman characteristics of M. flosaquae induced by different environmental conditions to assess the accumulation of the carotenoids in algae and to predict the algal growth phases.
关键词2-D Raman correlation spectroscopy accumulation phenomenon algal growth beta-carotene M flosaquae
DOI10.1002/jrs.5752
发表期刊JOURNAL OF RAMAN SPECTROSCOPY
ISSN0377-0486
页码10
通讯作者Du, Jinglei(dujl@scu.edu.cn) ; Du, Chunlei(cldu@cigit.ac.cn) ; Wang, Deqiang(dqwang@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:000495880600001
语种英语